3-D macro/microporous-nanofibrous bacterial cellulose scaffolds seeded with BMP-2 preconditioned mesenchymal stem cells exhibit remarkable potential for bone tissue engineering

Bone repair using BMP-2 is a promising therapeutic approach in clinical practices, however, high dosages required to be effective pose issues of cost and safety. The present study explores the potential low dose treatment via tissue engineering approach, which amalgamates 3-D macro/microporous-nanofibrous bacterial cellulose (mNBC) scaffolds primed murine mesenchymal stem cells (C3H10T1/2 cells). Initial studies on cell-scaffold interaction unprimed C3H10T1/2 confirmed that provided propitious environment for cell adhesion, growth, infiltration, owing its ECM-mimicking nano-micro-macro architecture. Osteogenic were conducted by preconditioning with 50 ng/mL 15 minutes, followed culturing mNBC up three weeks. results showed an early onset significantly enhanced bone matrix secretion maturation seeded compared ones. Moreover, alone able facilitate mineralization some extent. These findings suggest that, aid 'osteoinduction' from priming 'osteoconduction' nano-macro/micro topography scaffolds, cost-effective strategy can designed quick excellent vivo osseointegration.